brunello lentiviral library (Addgene inc)
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Addgene inc
brunello lentiviral library
Brunello Lentiviral Library, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 277 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/brunello+crispr+knockout+pooled+library/Human+CRISPR+Knockout+Pooled+Library+(Brunello)+(Pooled+Library+%2373179%2C+%2373179-LV%2C+%2373178%2C+%2373178-LV)/pm41875887-862-16-19
Average 96 stars, based on 277 article reviews
Brunello Lentiviral Library, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 277 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/brunello+crispr+knockout+pooled+library/Human+CRISPR+Knockout+Pooled+Library+(Brunello)+(Pooled+Library+%2373179%2C+%2373179-LV%2C+%2373178%2C+%2373178-LV)/pm41875887-862-16-19
Average 96 stars, based on 277 article reviews
brunello lentiviral library - by Bioz Stars,
2026-09
96/100 stars
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CRISPR:Article Title: Targeting the HTLV-I-Regulated BATF3/IRF4 Transcriptional Network in Adult T Cell Leukemia/Lymphoma Article Snippet: ATLL cell lines (ST1, KK1, Su9T01) and mantle cell lymphoma (MCL) lines were transduced in duplicate with the Brunello CRISPR knockout pooled library (gift of David Root and John Doench; Addgene #73178). .. ATLL cell lines (ST1, KK1, Su9T01) and mantle cell lymphoma (MCL) lines were transduced in duplicate with the Article Title: Targeting the HTLV-I-Regulated BATF3/IRF4 Transcriptional Network in Adult T Cell Leukemia/Lymphoma Article Snippet: .. CRISPR-Cas9 Screen Analysis ATLL cell lines (ST1, KK1, Su9T01) and mantle cell lymphoma (MCL) lines were transduced in duplicate with the Article Title: TP53-dependent toxicity of CRISPR/Cas9 cuts is differential across genomic loci and can confound genetic screening Article Snippet: .. Moreover the genetic screening data on the A549 TP53wt and TP53 −/− cells (the pseudoreplicates with and without doxycycline) was as reported in Biayna et al. , from where we quote verbatim “For sgRNA screening of the A549 [...], A549 TP53 −/− [...], cells were infected with the Article Title: Targeting the HTLV-I-Regulated BATF3/IRF4 Transcriptional Network in Adult T Cell Leukemia/Lymphoma Article Snippet: LentiCas9-Blast , A gift from Dr. Feng Zhang (Addgene plasmid) , # 52962. .. Article Title: Genome-wide CRISPR screen identifies CDK6 as a therapeutic target in adult T-cell leukemia/lymphoma. Article Snippet: .. Cell lines and cell culture All lines were cultured with RPMI media containing 10% fetal calf serum, penicillin, and streptomycin and were maintained in a humidified, 5% CO2 incubator at 37 C. Whole-genome CRISPR-Cas9 screen analysis Single-guide RNA (sgRNA) library screening was performed as previously described.5 Cell lines were transduced in duplicate with the Article Title: Loss of the abasic site sensor HMCES is synthetic lethal with the activity of the APOBEC3A cytosine deaminase in cancer cells. Article Snippet: The cell lines were transduced and selected with puromycin for 72 h. As a control, we transduced LXF-289 (A3A) cells with the nonmammalian shRNA Control Plasmid DNA shC002 (MilliporeSigma). .. For sgRNA screening of the A549 ± A3A, A549TP53−/− ± A3A, LXF-289 ± A3A, cells were infected with the Article Title: Whole-genome CRISPR screening identifies molecular mechanisms of PD-L1 expression in adult T-cell leukemia/lymphoma Article Snippet: .. ATLL cell lines, ST1 and KK1, were transduced with Knock-Out:Article Title: Targeting the HTLV-I-Regulated BATF3/IRF4 Transcriptional Network in Adult T Cell Leukemia/Lymphoma Article Snippet: ATLL cell lines (ST1, KK1, Su9T01) and mantle cell lymphoma (MCL) lines were transduced in duplicate with the Brunello CRISPR knockout pooled library (gift of David Root and John Doench; Addgene #73178). .. ATLL cell lines (ST1, KK1, Su9T01) and mantle cell lymphoma (MCL) lines were transduced in duplicate with the Article Title: Targeting the HTLV-I-Regulated BATF3/IRF4 Transcriptional Network in Adult T Cell Leukemia/Lymphoma Article Snippet: .. CRISPR-Cas9 Screen Analysis ATLL cell lines (ST1, KK1, Su9T01) and mantle cell lymphoma (MCL) lines were transduced in duplicate with the Article Title: TP53-dependent toxicity of CRISPR/Cas9 cuts is differential across genomic loci and can confound genetic screening Article Snippet: .. Moreover the genetic screening data on the A549 TP53wt and TP53 −/− cells (the pseudoreplicates with and without doxycycline) was as reported in Biayna et al. , from where we quote verbatim “For sgRNA screening of the A549 [...], A549 TP53 −/− [...], cells were infected with the Article Title: Targeting the HTLV-I-Regulated BATF3/IRF4 Transcriptional Network in Adult T Cell Leukemia/Lymphoma Article Snippet: LentiCas9-Blast , A gift from Dr. Feng Zhang (Addgene plasmid) , # 52962. .. Article Title: Genome-wide CRISPR screen identifies CDK6 as a therapeutic target in adult T-cell leukemia/lymphoma. Article Snippet: .. Cell lines and cell culture All lines were cultured with RPMI media containing 10% fetal calf serum, penicillin, and streptomycin and were maintained in a humidified, 5% CO2 incubator at 37 C. Whole-genome CRISPR-Cas9 screen analysis Single-guide RNA (sgRNA) library screening was performed as previously described.5 Cell lines were transduced in duplicate with the Article Title: Loss of the abasic site sensor HMCES is synthetic lethal with the activity of the APOBEC3A cytosine deaminase in cancer cells. Article Snippet: The cell lines were transduced and selected with puromycin for 72 h. As a control, we transduced LXF-289 (A3A) cells with the nonmammalian shRNA Control Plasmid DNA shC002 (MilliporeSigma). .. For sgRNA screening of the A549 ± A3A, A549TP53−/− ± A3A, LXF-289 ± A3A, cells were infected with the Article Title: Whole-genome CRISPR screening identifies molecular mechanisms of PD-L1 expression in adult T-cell leukemia/lymphoma Article Snippet: .. ATLL cell lines, ST1 and KK1, were transduced with Infection:Article Title: TP53-dependent toxicity of CRISPR/Cas9 cuts is differential across genomic loci and can confound genetic screening Article Snippet: .. Moreover the genetic screening data on the A549 TP53wt and TP53 −/− cells (the pseudoreplicates with and without doxycycline) was as reported in Biayna et al. , from where we quote verbatim “For sgRNA screening of the A549 [...], A549 TP53 −/− [...], cells were infected with the Article Title: Loss of the abasic site sensor HMCES is synthetic lethal with the activity of the APOBEC3A cytosine deaminase in cancer cells. Article Snippet: The cell lines were transduced and selected with puromycin for 72 h. As a control, we transduced LXF-289 (A3A) cells with the nonmammalian shRNA Control Plasmid DNA shC002 (MilliporeSigma). .. For sgRNA screening of the A549 ± A3A, A549TP53−/− ± A3A, LXF-289 ± A3A, cells were infected with the Plasmid Preparation:Article Title: Targeting the HTLV-I-Regulated BATF3/IRF4 Transcriptional Network in Adult T Cell Leukemia/Lymphoma Article Snippet: LentiCas9-Blast , A gift from Dr. Feng Zhang (Addgene plasmid) , # 52962. .. Cell Culture:Article Title: Genome-wide CRISPR screen identifies CDK6 as a therapeutic target in adult T-cell leukemia/lymphoma. Article Snippet: .. Cell lines and cell culture All lines were cultured with RPMI media containing 10% fetal calf serum, penicillin, and streptomycin and were maintained in a humidified, 5% CO2 incubator at 37 C. Whole-genome CRISPR-Cas9 screen analysis Single-guide RNA (sgRNA) library screening was performed as previously described.5 Cell lines were transduced in duplicate with the Library Screening:Article Title: Genome-wide CRISPR screen identifies CDK6 as a therapeutic target in adult T-cell leukemia/lymphoma. Article Snippet: .. Cell lines and cell culture All lines were cultured with RPMI media containing 10% fetal calf serum, penicillin, and streptomycin and were maintained in a humidified, 5% CO2 incubator at 37 C. Whole-genome CRISPR-Cas9 screen analysis Single-guide RNA (sgRNA) library screening was performed as previously described.5 Cell lines were transduced in duplicate with the Transduction:Article Title: Whole-genome CRISPR screening identifies molecular mechanisms of PD-L1 expression in adult T-cell leukemia/lymphoma Article Snippet: .. ATLL cell lines, ST1 and KK1, were transduced with |
